Custom Agarose Particles

Agarose particles¡ªporous hydrogel microspheres derived from red algae¡ªhave become indispensable tools in bioseparation and biomedical research. Their exceptional biocompatibility, hydrophilicity, and tunable porosity make them ideal supports for affinity, ion exchange, and size-exclusion chromatography. However, standard off-the-shelf particles cannot always meet the demands of specialized workflows¡ªwhether it's optimizing particle size for resolution versus flow rate, adjusting pore architecture for specific molecular targets, or introducing custom ligands for unique capture needs. This is where custom agarose particles come into play.

The Versatility of Agarose Particles for Biomolecule Purification

Agarose particles are porous gel microspheres that separate mixed biomolecules based on their molecular sizes. They are easily activated and compatible with both batch incubation and column purification workflows for proteins and antibodies. The abundant internal porous structure enables efficient purification of large biomolecules such as monoclonal antibodies.

After being coupled with functional groups, proteins or antibodies on the surface, agarose particles can capture target substances via centrifugation or magnetic separation. They can be applied as affinity chromatography resins, ion exchange chromatography resins, size-exclusion chromatography resins, and hydrophobic interaction chromatography resins.

Properties of Agarose Particles

¡¤ High purity of purified target proteins

¡¤ High recovery rate after elution

¡¤ Reusable for multiple cycles

¡¤ Excellent chemical stability

¡¤ High binding capacity

¡¤ Easy activation process

Applications of Agarose Particles

¡¤ Stationary supports for affinity chromatography, ion exchange chromatography and hydrophobic interaction chromatography

¡¤ Core reagents for chromatin immunoprecipitation (ChIP), immunoprecipitation (IP) and pull-down assays

¡¤ Materials for antibody fragmentation

¡¤ Purification carriers for tagged proteins and antibodies

¡¤ Conjugation matrix for antibodies, streptavidin, enzymes and nucleic acids

¡¤ Raw materials for biopharmaceutical and bioengineering industrial preparation

Why BOT Bioparticles£¿

BOT Bioparticles provides comprehensive custom services including agarose particle synthesis, surface functionalization and biomolecule conjugation. Apart from our cost-effective standard agarose products, we deliver tailor-made solutions to satisfy personalized research and industrial demands. Supported by mature technologies and advanced equipment, we can conduct various modifications and conjugations on agarose particles with antibodies, proteins, peptides and other biomolecules as requested. Custom magnetic agarose particles for rapid magnetic separation are also available, and bulk orders are supported.

What Does BOT Bioparticles¡¯ Custom Service Provide?

Multiple adjustable parameters are available to build exclusive agarose particle systems, covering physical specifications and surface modification types.

1. Base adjustable parameters

¡¤ Matrix type: Magnetic agarose particles or non-magnetic agarose particles

¡¤ Particle diameter: 30¨C800 ¦Ìm

¡¤ Custom agarose percentage

¡¤ Spacer arm length: C0 to C6 and longer chains

2. Two core custom modification categories 

(1) Functionalization (surface reactive group modification) Carboxyl, Amine, Maleimide, DBCO, Ni-NTA, Azide, Alkyne, NHS and other chemical groups

(2) Conjugation (coupling with biomolecules or small-molecule drugs)

¡¤ Protein: Protein A, Protein G, Streptavidin

¡¤ Antibody: Anti-His Tag Antibody, Anti-Rho1D4 Antibody and customized antibodies

¡¤ Peptide: Exendin, Glutathione and self-designed peptides

¡¤ Small Molecule Drug: Alprenolol, Paroxetine and other small ligands

How to Start Your Project?

To launch your custom agarose particle project, please provide the following information by filling out our 

Custom Agarose Particles-Questionnaire.pdf

l Requirement for magnetic or non-magnetic particles

l Target particle diameter

l Required agarose percentage

l Spacer arm length demand

l Surface modification type: functionalization or conjugation

l Total production quantity

l Suspension format: Standard bulk resins are 50% suspension; magnetic particles are 20% suspension. Custom concentrations or pure dry agarose particles are acceptable.

l Special QC testing requirements

After submitting the Questionnaire, you can contact our team at info@bot-bioscience.com to discuss technical details, request quotations or obtain customized technical proposals for agarose particle construction services.