Magnetic Beads-Oligonucleotide Conjugates Services

SERVICE NO: TSS045

Particle conjugation has been used in various biological applications. Biomolecules include antibodies, protein A/G, lectins, enzymes, toxins, and other proteins have all been conjugated to macroparticle or nanoparticle for diagnostic tests. Nanoparticle and microparticles are used in agglutination tests and assays, particle capture ELISA methods, lateral flow tests, solid phase assays, scintillation proximity assays, PCR, superparamagnetic-based assays and magnetic separation systems, biosensors, as enhancers of Ramam spectral signals, in light scattering assays, and as fluorescent labels or stains for detecting biological molecules. 

Among them, Magnetic beads are used for separation procedures to enrich the population of a specific cell, or isolate biomolecules for purification purposes. Magnetic beads/particles are typically used as solid supports for the capture of DNA targets to improve sample throughput traditionally. However, it aggregate over time resulting in lower capture efficiency and obstruction of liquid handling devices. A new approach is developed-using magnetic beads and tagged oligonucleotide (ITO) probes. ITO and magnetic approach was more sensitive than a commercial magnetic bead method for the capture of target DNA from a pool of interfering genomic DNA. 

(1)Progress of Conjugation

Activation of magnetic beads

Coupling reaction of probes and magnetic beads

Blocking active groups

Preservation

(2)Other Bioconjugation Portfolios: 

Agarose-DNA

Agarose-Peptide

Oligo-qDot

Antibody/antigen-membrane

DNA-Magnetic Beads

DNA-Gold nanoparticles

Antibody-Gold

Lecint-Gold Conjugates

(Strep)avidin-Gold Conjugates

Oligonucleotide-Gold Conjugates

Fluorescent Labeled Latex

High binding capacity

Cost-effective

Less non-specific adsorption

High quality well-characterized conjugates

Flexible service options

The experience and ability of a dedicated team